In vitro versus in vivo method
Classical medicine uses mainly in vitro methods (wet analysis) to determine various blood parameters. The in vitro method, although it has fit into the ‘gold standard’ position, as it now appears, has a huge standardization problem. These problems of standardizing “in vitro” analytical methods were also presented by participants at this year’s International Symposium in Barcelona (VIII. Simpósium Europeu). Participants from different countries very openly stated that achieving standardization, even in one larger hospital, is quite difficult, even impossible. Why? It should be noted that innumerable factors, both known and unknown, affect the accuracy and veracity of the result measured by the in vitro method. Their complete elemination is virtually impossible. The effect of these factors is manifested in the considerable variability of the analysis results in inter- and intra-comparative studies. However, much of these known and unknown factors can be eliminated today by new measurement methods that are now being introduced into medicine. Optical or electro-impedance methods for measuring blood parameters and systems are now commonly used.
From the viewpoint of analytical chemistry in an in vivo measurement, a certain set of possible errors that are commonly encountered in the in vitro method and which is summarized under the accuracy of the result is eliminated. Accuracy of results is often influenced by in vitro method of measuring any parameter in blood often by accuracy and accuracy of sampling, time between collection and time of measurement, transport and storage of sample, accuracy and accuracy of measurement in a huge mixture of substances unknown to us. parameter measurement process, reagent quality, calibration, personnel quality, etc. In practice, all these ills of the in vitro method in the in vivo method of measuring blood parameters are eliminated. Clinical investigations confirmed that non-invasive blood analysis using the AMP analyzer (ANESA) statistically demonstrated nearly 100% reproducibility of the parameter measurements alone and a high compliance with the in vitro measured parameters. The measurement method itself is very simple, itself. Thus, the in vivo method of measuring blood parameters using a non-invasive AMP (ANESA) analyzer predisposes the device primarily for first-contact physicians but also for routine analysis to monitor the development of pathology or the quality of therapy in clinics or hospitals. What is important to know at the end: super knowledge of human physiology.